The sensitivity of cells from normal mouse bone marrow to gamma radiation in vitro and in vivo.

@article{McCulloch1962TheSO,
  title={The sensitivity of cells from normal mouse bone marrow to gamma radiation in vitro and in vivo.},
  author={E. McCulloch and J. Till},
  journal={Radiation research},
  year={1962},
  volume={16},
  pages={
          822-32
        }
}
1. A survival curve for the colony-forming ability of mouse bone marrow cells irradiated in vivo with Co60 gamma-rays has been obtained. The curve may be characterized by D0 of 95 rads and an extrapolation number of 1.5. 2. Comparison with the survival curve obtained for irradiated in vitro indicated a statistically significant difference in extrapolation number. Possible explanations for this finding are considered. 
Radiation sensitivity of spleen cells irradiated in vitro and in vivo.
X-irradiation survival response by colony-forming ability was determined for mouse spleen cells. Cells irradiated in vitro or in vivo differed in their radiosensitivity and cells irradiated in vivoExpand
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The response of bone marrow stem cells to repeated large doses of x-rays was investigated in the mouse using the spleen colony assay technique, and reduced recovery rates are shown to be due to damage to the milieu rather thandamage to the cells themselves. Expand
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Recuperation of the ability of irradiated mouse bone marrow to support clonal growth was observed at intervals of 9 and 12 months and the proliferation ratio did not decrease below 0.28 even when the bone marrow had received as much as 3000 rad. Expand
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This small animal model may prove amenable for the analysis of the risk of the exposure of humans to radiation as well as for the development of new modalities for the prevention and treatment of radiation-induced hematopoietic damage. Expand
REPAIR PROCESSES IN IRRADIATED MOUSE HEMATOPOIETIC TISSUE *
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It was found that radiosensitivity of endogenous colony-forming cells is similar to that of transplanted coIony-forming Cells, and late repair processes commencing at 24 hr postirradiation were found to increase with time intervals up to 14 days between radiation doses, but to decrease between 14 and 20 days. Expand
Early repair processes in marrow cells irradiated and proliferating in vivo.
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An assay procedure that may be used for the study of the behavior of spleen-colonizing cells in situ was developed and indicated that early repair reached an initial maximum at about 5 hours after the first dose fraction, followed by an intermediate minimum at about 11 hours. Expand
Dose rate effects on the survival of normal hematopoietic stem cells of BALB/c mice.
TLDR
D dose rate effects at 103, 45 and 8 rad/min over the dose range from 100 to 500 rad are investigated and there are no statistically significant differences between the n's and D0's for these different dose rates as measured by spleen CFU assay of normal femoral marrow. Expand
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It is suggested that RES-blockaded mice provide a favorable microenvironment for the recovery of hemopoietic stem cells after irradiation. Expand
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A shallow dose-related decrease in the ability of irradiated mouse spleen to support clonal growth was seen at all times after splenic irradiation, and dose-response relationships do not resemble the usual clonogenic survival curves. Expand
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TLDR
The effectiveness of 14 MeV neutrons from the D-T reaction was compared with that of 60Co γ-radiation in killing mouse bone-marrow cells capable of forming spleen colonies after transfer to lethally-irradiated isologous recipients to find the relative biological effectiveness (RBE) was 1·67 ± 0·06. Expand
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