The sensitivity of cells from normal mouse bone marrow to gamma radiation in vitro and in vivo.

  title={The sensitivity of cells from normal mouse bone marrow to gamma radiation in vitro and in vivo.},
  author={E. McCulloch and J. Till},
  journal={Radiation research},
1. A survival curve for the colony-forming ability of mouse bone marrow cells irradiated in vivo with Co60 gamma-rays has been obtained. The curve may be characterized by D0 of 95 rads and an extrapolation number of 1.5. 2. Comparison with the survival curve obtained for irradiated in vitro indicated a statistically significant difference in extrapolation number. Possible explanations for this finding are considered. 
Radiation sensitivity of spleen cells irradiated in vitro and in vivo.
X-irradiation survival response by colony-forming ability was determined for mouse spleen cells. Cells irradiated in vitro or in vivo differed in their radiosensitivity and cells irradiated in vivoExpand
The response of hemopoietic colony-forming units to repeated doses of x-rays.
The response of bone marrow stem cells to repeated large doses of x-rays was investigated in the mouse using the spleen colony assay technique, and reduced recovery rates are shown to be due to damage to the milieu rather thandamage to the cells themselves. Expand
Late effects of x-irradiation on the ability of mouse bone marrow to support hematopoiesis.
Recuperation of the ability of irradiated mouse bone marrow to support clonal growth was observed at intervals of 9 and 12 months and the proliferation ratio did not decrease below 0.28 even when the bone marrow had received as much as 3000 rad. Expand
Direct evaluation of radiation damage in human hematopoietic progenitor cells in vivo.
This small animal model may prove amenable for the analysis of the risk of the exposure of humans to radiation as well as for the development of new modalities for the prevention and treatment of radiation-induced hematopoietic damage. Expand
It was found that radiosensitivity of endogenous colony-forming cells is similar to that of transplanted coIony-forming Cells, and late repair processes commencing at 24 hr postirradiation were found to increase with time intervals up to 14 days between radiation doses, but to decrease between 14 and 20 days. Expand
Early repair processes in marrow cells irradiated and proliferating in vivo.
An assay procedure that may be used for the study of the behavior of spleen-colonizing cells in situ was developed and indicated that early repair reached an initial maximum at about 5 hours after the first dose fraction, followed by an intermediate minimum at about 11 hours. Expand
Dose rate effects on the survival of normal hematopoietic stem cells of BALB/c mice.
D dose rate effects at 103, 45 and 8 rad/min over the dose range from 100 to 500 rad are investigated and there are no statistically significant differences between the n's and D0's for these different dose rates as measured by spleen CFU assay of normal femoral marrow. Expand
Recovery of irradiated bone marrow cells in carbon-treated mice.
It is suggested that RES-blockaded mice provide a favorable microenvironment for the recovery of hemopoietic stem cells after irradiation. Expand
The long-term effects of x-irradiation on the ability of the spleen to support clonogenic proliferation.
A shallow dose-related decrease in the ability of irradiated mouse spleen to support clonal growth was seen at all times after splenic irradiation, and dose-response relationships do not resemble the usual clonogenic survival curves. Expand
The RBE of 14 MeV neutrons. Observations on colony-forming units in mouse bone-marrow.
The effectiveness of 14 MeV neutrons from the D-T reaction was compared with that of 60Co γ-radiation in killing mouse bone-marrow cells capable of forming spleen colonies after transfer to lethally-irradiated isologous recipients to find the relative biological effectiveness (RBE) was 1·67 ± 0·06. Expand


The radiation sensitivity of normal mouse bone marrow cells, determined by quantitative marrow transplantation into irradiated mice.
Quantitative bone marrow transplantation was used to obtain an estimate of the radiation sensitivity of normal mouse bone marrow progenitor cells, and this estimate was lower than expected because fewer cells were transplanted. Expand
A direct measurement of the radiation sensitivity of normal mouse bone marrow cells.
Counts of macroscopic splenic colonies were used to obtain an estimate of the radiation sensitivity of normal mouse bone marrow progenitor cells. Reproduced from Radiation Research 1961(Feb); 14(2):Expand
The results of in vivo studies on irradiated tumor cells were surveyed and it was concluded that they are reassuring, in the sense in which they show that the curves obtained by irradiation of cellsExpand
Radiation response of mammalian cells grown in culture. 1. Repair of X-ray damage in surviving Chinese hamster cells.
A study was undertaken, using two-cell lines of the Chinese hamster propagated in vitro, to determine whether or not sublethal damage is heritsble, as might be expected if lethal damage is primarily genic. Expand
The relationship between growth and survival after irradiation of Escherichia coli strain B and two resistant mutants.
Faster growth of Escherichia coli strain B cells is observed in media which contain factors previously found to increase the numbers killed by radiation, an exception to the principle that greater survival of strain B is associated with slower growth. Expand
The Sigmoid Survival Curve in Radiobiology
The form of survival curve illustrated by Fig. 1 (which would be sigmoid on a linear plot) has been established, with varying degrees of accuracy, for many single-cell populations, ranging from bacteria to mammalian cells. Expand
Histopathologic Technic and Practical Histochemistry
Histopathologies technic and practical histochemistry, Histopathologie techno-practical histochemistry and the role of nanofiltration in medicine and drug discovery and abuse are studied. Expand
MCCULLOCH, A direct measurement of the radiation sensitivity of normal mouse bone marrow cells
  • Radiation Research
  • 1961
SUTTON, Radiation response of mamalian cells grown in culture
  • Radiation Research
  • 1960
GRAY, Cellular radiobiology
  • Radiation Research Suppl
  • 1959