The selective detection of mitochondrial superoxide by live cell imaging

  title={The selective detection of mitochondrial superoxide by live cell imaging},
  author={Kristine M. Robinson and Michael S. Janes and Joseph S. Beckman},
  journal={Nature Protocols},
A general protocol is described to improve the specificity for imaging superoxide formation in live cells via fluorescence microscopy with either hydroethidine (HE) or its mitochondrially targeted derivative Mito-HE (MitoSOX Red). Two different excitation wavelengths are used to distinguish the superoxide-dependent hydroxylation of Mito-HE (385–405 nm) from the nonspecific formation of ethidium (480–520 nm). Furthermore, the dual wavelength imaging in live cells can be combined with… CONTINUE READING
Highly Influential
This paper has highly influenced 11 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

From This Paper

Figures, tables, and topics from this paper.


Publications citing this paper.
Showing 1-10 of 95 extracted citations

Mitochondrial ATP-Mg/Pi carrier SCaMC-3/Slc25a23 counteracts PARP-1-dependent fall in mitochondrial ATP caused by excitotoxic insults in neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience • 2015
View 8 Excerpts
Highly Influenced

The specificity of neuroprotection by antioxidants

Journal of Biomedical Science • 2009
View 3 Excerpts
Highly Influenced


Publications referenced by this paper.
Showing 1-10 of 18 references

identification of homo- and heterodimers

Zielonka, J. et al. Cytochrome c-mediated oxidation of hydroethidine, mito-hydroethidine in mitochondria
Free Radic. Biol. Med. 44, 835–846 • 2008

Multiparametric analysis of cells with different mitochondrial membrane potential during apoptosis by polychromatic flow cytometry

L Troiano
Nat. Protoc • 2007

Selective fluorescent imaging of superoxide in vivo using ethidium-based probes.

Proceedings of the National Academy of Sciences of the United States of America • 2006

Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence

H Zhao
Proc. Natl. Acad. Sci. USA 102, • 2005

Similar Papers

Loading similar papers…