The selective detection of mitochondrial superoxide by live cell imaging

@article{Robinson2008TheSD,
  title={The selective detection of mitochondrial superoxide by live cell imaging},
  author={Kristine M. Robinson and Michael S. Janes and Joseph S. Beckman},
  journal={Nature Protocols},
  year={2008},
  volume={3},
  pages={941-947}
}
A general protocol is described to improve the specificity for imaging superoxide formation in live cells via fluorescence microscopy with either hydroethidine (HE) or its mitochondrially targeted derivative Mito-HE (MitoSOX Red). Two different excitation wavelengths are used to distinguish the superoxide-dependent hydroxylation of Mito-HE (385–405 nm) from the nonspecific formation of ethidium (480–520 nm). Furthermore, the dual wavelength imaging in live cells can be combined with… CONTINUE READING
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