The selective and inducible activation of endogenous PI 3-kinase in PC12 cells results in efficient NGF-mediated survival but defective neurite outgrowth

@article{Ashcroft1999TheSA,
  title={The selective and inducible activation of endogenous PI 3-kinase in PC12 cells results in efficient NGF-mediated survival but defective neurite outgrowth},
  author={Margaret Ashcroft and Robert M. Stephens and Bengt Hallberg and Julian Downward and David R Kaplan},
  journal={Oncogene},
  year={1999},
  volume={18},
  pages={4586-4597}
}
The Trk/Nerve Growth Factor receptor mediates the rapid activation of a number of intracellular signaling proteins, including phosphatidylinositol 3-kinase (PI 3-kinase). Here, we describe a novel, NGF-inducible system that we used to specifically address the signaling potential of endogenous PI 3-kinase in NGF-mediated neuronal survival and differentiation processes. This system utilizes a Trk receptor mutant (Trkdef) lacking sequences Y490, Y785 and KFG important for the activation of the… CONTINUE READING

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