The scaffold protein Ste5 directly controls a switch-like mating decision in yeast

  title={The scaffold protein Ste5 directly controls a switch-like mating decision in yeast},
  author={Mohan Malleshaiah and Vahid Shahrezaei and Peter S. Swain and Stephen W. Michnick},
Evolution has resulted in numerous innovations that allow organisms to increase their fitness by choosing particular mating partners, including secondary sexual characteristics, behavioural patterns, chemical attractants and corresponding sensory mechanisms. [] Key Result This competition results in a switch-like dissociation of Fus3 from Ste5 that is necessary to generate the switch-like mating response.

Utilization of the Mating Scaffold Protein in the Evolution of a New Signal Transduction Pathway for Biofilm Development

It is demonstrated that Cst5, a homolog of the Saccharomyces cerevisiae scaffold protein Ste5, functions as the scaffoldprotein in both the opaque and the white cell pheromone responses, supporting the evolutionary scenario proposed.

Stochastic model of mating decision control in yeast through the scaffold protein Ste5

The model of a switch-like mating decision in the haploid yeast S. cerevisiae was modified in order to perform both deterministic and stochastic analysis, and the system was not affected by kinetic rate changes and thereby robust.

Conformational Control of the Ste5 Scaffold Protein Insulates Against MAP Kinase Misactivation

It is shown that the scaffold protein Ste5, which organizes the yeast mating mitogen-activated protein kinase (MAPK) pathway, does not use sequestration to prevent misactivation of the mating response, and appears to use a conformation mechanism.

Mate and fuse: how yeast cells do it

This review presents the current knowledge on the processes of mating signalling, pheromone-dependent polarized growth and cell fusion in Saccharomyces cerevisiae and Schizosac charomyces pombe, two highly divergent ascomycete yeast models.

Mathematical modeling for multisite phosphorylation with scaffold binding in cell signaling

It is argued that the scaffold protein plays an important role in creating bistability, and the complexity of calculating steady states from simulating differential equations to finding the roots of polynomials is reduced by developing generic mathematical models.

Signal-mediated localization of Candida albicans pheromone response pathway components

GFP-tagged fusion proteins are used to investigate the localization of the scaffold protein Cst5, as well as the MAP kinases Cek1 and Cek2, during pheromone response in C. albicans and results are consistent with CaSte11 facilitating Hst7-mediated MAP kinase phosphorylation and also playing a potentially critical role in bothMAP kinase and Cst 5 scaffold localization.

Sensory input attenuation allows predictive sexual response in yeast

It is demonstrated that pheromone-based communication between the two mating types, coupled to input attenuation by recipient cells, enables yeast to robustly monitor relative mate abundance (sex ratio) within a mixed population and to adjust their commitment to sexual reproduction in proportion to their estimated chances of successful mating.

Reconstructing the regulatory circuit of cell fate determination in yeast mating response

The mating differentiation in yeast Saccharomyces cerevisiae is used as a model and integrated experimental and computational approaches are developed to analyze the proteomic dynamics during the process of cell fate determination to address the distinct impacts of the global and gene-specific regulation on the control of protein dynamics.

Dynamic modeling of yeast meiotic initiation

It is argued that the architecture of meiotic initiation pathway generates a robust mechanism that assures a rapid and complete transition into meiosis, and key regulations that can be manipulated to enhance sporulation efficiency are uncovered.



A filamentous growth response mediated by the yeast mating pathway.

It is suggested that mating yeast cells undergo a complex response to low levels of pheromone that may enhance the ability of cells to search for mating partners through the modification of cell shape and alteration of cell-division patterns.

MAPK-mediated bimodal gene expression and adaptive gradient sensing in yeast

Both the switch-like characteristics and sensitivity of gene expression in shmooing cells to pheromone concentration were significantly diminished in cells lacking Kss1, one of the MAP kinases activated in the mating pathway, providing unsuspected functional significance for this kinase in regulation of the mating response.

Pheromone response, mating and cell biology.

  • E. Elion
  • Biology
    Current opinion in microbiology
  • 2000

The Ste5 Scaffold Allosterically Modulates Signaling Output of the Yeast Mating Pathway

It is found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3 appears to have a negative regulatory role.

Cross-talk and decision making in MAP kinase pathways

It is shown that in a single cell, the hyperosmolar and pheromone mitogen-activated protein (MAP) kinase pathways in the yeast Saccharomyces cerevisiae are bistable over a range of inputs, and the cell responds to only one stimulus even when exposed to both.

Ptc1, a Type 2C Ser/Thr Phosphatase, Inactivates the HOG Pathway by Dephosphorylating the Mitogen-Activated Protein Kinase Hog1

It is shown that PTC2 also suppresses sln1Δ lethality, and one function of PTC1 is to inactivate Hog1, which accumulates in the nucleus upon activation and is found in both the nucleus and the cytoplasm.