The ryanodine receptor from canine heart sarcoplasmic reticulum is associated with a novel FK-506 binding protein.

@article{Timerman1994TheRR,
  title={The ryanodine receptor from canine heart sarcoplasmic reticulum is associated with a novel FK-506 binding protein.},
  author={A P Timerman and Thottala Jayaraman and Gregory J Wiederrecht and Hitoshi Onoue and Andrew Robert Marks and Sidney Fleischer},
  journal={Biochemical and biophysical research communications},
  year={1994},
  volume={198 2},
  pages={701-6}
}
We find that the purified ryanodine receptor/calcium release channel (CRC) isolated from canine heart sarcoplasmic reticulum (SR) is tightly associated with an FK-506 binding protein which is distinct from human recombinant FKBP-12. Fractions from both skeletal and heart muscle were probed by Western Blot analysis using sequence specific antibody raised against the N-terminus of human recombinant FKBP-12. Only FKBP-12 was detected in rabbit and dog skeletal muscle SR as well as in dog heart… CONTINUE READING