• Corpus ID: 54644981

The role of thymidine kinase 1 in preferential DNA repair

  title={The role of thymidine kinase 1 in preferential DNA repair},
  author={Sebastien A Rider and James Reeves and Kenneth Armstrong and Jan and Knight and S J Davies and Kristine Bjerve Gutzkow and Torgrim Mikal Langleite and Ville Erling Sipinen and Andrew R. Collins}


Mutation Research/Genetic Toxicology and Environmental Mutagenesis
The possible genotoxic,cytotoxic and aneugenic effects caused by this agent in different stages of the cell cycle using different cytogenetic parameters are determined in lymphocytes from temporary cultures of humanipheralblood.
UVA-induced apoptosis studied by the new apo/necro-Comet-assay which distinguishes viable, apoptotic and necrotic cells.
An adaptation of the Comet-assay was developed which enables the discrimination of viable, apoptotic and necrotic single cells by use of the common Annexin-V staining and a dye exclusion test on the cells already embedded in agarose gel on glass slides, and showed that there was no significant interference of DNA repair by apoptotic fragmentation processes since DNA repair was almost completed before the onset of apoptosis.
Metal-specific differences in levels of DNA damage caused by synovial fluid recovered at revision arthroplasty.
The metal-specific difference in the level of DNA damage induced by culturing human fibroblasts in synovial fluid retrieved at revision arthroplasty is investigated and it is emphasised that epidemiological studies of orthopaedic implants should take account of the type of metal alloy used.