The role of the loxP spacer region in P1 site-specific recombination.

  title={The role of the loxP spacer region in P1 site-specific recombination.},
  author={Ronald H. Hoess and Anna Wierzbicki and Ken Abremski},
  journal={Nucleic acids research},
  volume={14 5},
The lox-Cre site-specific recombination system of bacteriophage P1 is comprised of a site on the DNA where recombination occurs called loxP, and a protein, Cre, which mediates the reaction. The loxP site is 34 base pairs (bp) in length and consists of two 13 bp inverted repeats separated by an 8 bp spacer region. Previously it has been shown that the cleavage and strand exchange of recombining loxP sites occurs within this spacer region. We report here an analysis of various base substitution… CONTINUE READING

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