The role of the essential sulfhydryl group in assimilatory NADH: nitrate reductase of Chlorella.

@article{Barber1986TheRO,
  title={The role of the essential sulfhydryl group in assimilatory NADH: nitrate reductase of Chlorella.},
  author={Michael J. Barber and Larry P. Solomonson},
  journal={The Journal of biological chemistry},
  year={1986},
  volume={261 10},
  pages={4562-7}
}
Incubation of the complex metalloflavoprotein, assimilatory nitrate reductase with N-ethylmaleimide, or a spin-labeled analog, 4-maleimido-2,2,6,6-tetramethylpiperidinooxyl, resulted in a time-dependent inactivation of NADH:nitrate reductase and NADH: cytochrome-c reductase activity with no effect on reduced methyl viologen:nitrate reductase activity. Inactivation of the enzyme, which could be prevented by incubation in the presence of NADH, was achieved following modification of a single… CONTINUE READING

References

Publications referenced by this paper.

Polyhedron, in press

L. P. Solomonson, M. J. Barber, W. D. Howard, J. L. Johnson, K. V. Rajagopalan
Annu. Rev. Plant Physiol • 1984

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