The role of the Aspergillus fumigatusareA gene in invasive pulmonary aspergillosis

  title={The role of the Aspergillus fumigatusareA gene in invasive pulmonary aspergillosis},
  author={Michael T. Hensel and Herbert N. Arst, Jr and Agn{\`e}s Aufauvre‐Brown and David W. Holden},
  journal={Molecular and General Genetics MGG},
Abstract The areA gene of Aspergillus nidulans is a positive-acting transcriptional factor required for the expression of genes involved in the utilization of a broad range of nitrogen sources other than ammonium and glutamine. We have investigated the role in pathogenesis of the corresponding gene (AfareA) of Aspergillus fumigatus, a causative agent of invasive pulmonary aspergillosis. Stable and unstable AfareA− strains were constructed and tested for altered virulence in mice on the basis of… 

Identification of genes of Aspergillus fumigatus up-regulated during growth on endothelial cells.

A tissue culture model was developed to mimic the interaction of the fungus with the endothelium and provides a new approach to the identification of potentially virulence-related genes induced in A. fumigatus by the interaction with host cells.

Determining Aspergillus fumigatus transcription factor expression and function during invasion of the mammalian lung

The transcriptional profiling data indicate that the organism is exposed to nutrient limitation and stress during growth in the lungs, and that it responds by up-regulating genes that encode mycotoxins and secondary metabolites.

Putative virulence factors of Aspergillus fumigatus

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  • Biology
    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
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None of the reports demonstrates conclusively a decisive role for any of the virulence factors described thus far, but it is conceivable that proteolytic enzyme activities such as those expressed by AFAlp are one of a number of factors that combine to facilitate disease progression.

Pathogenesis of Aspergillus fumigatus in Invasive Aspergillosis

An overview of the significant findings regarding A. fumigatus pathogenesis as it pertains to invasive disease is provided.

A novel method used to delete a new Aspergillus fumigatus ABC transporter-encoding gene

Here, it is identified a new ABC transporter-encoding gene in A. fumigatus, designated abcA, providing evidence that this method of generating gene deletions can also be used in Aspergillus fumgatus and significantly broadens the repertoire of molecular techniques to study A.fumigatu.

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Role of Amino Acid Metabolism in the Virulence of Human Pathogenic Fungi

Although many components of amino acid sensing and metabolism are fungal specific, their importance in infection and potential as candidates for antifungal drug development require further investigation.

Olorofim and the azoles are antagonistic in A. fumigatus and functional genomic screens reveal mechanisms of cross resistance.

Transcriptomic analysis suggests that altered sensitivity to olorofim may emerge via modification of genes involved in the production of pyrimidine biosynthetic precursors in A. fumigatus, and it is shown that the action of the azoles are antagonistic to oloofim in vitro.

Aspergillus fumigatus and Aspergillosis in 2019

The emergence of new concepts, such as nutritional immunity and the integration and rewiring of multiple fungal metabolic activities occurring during lung invasion, has helped to redefine the opportunistic pathogenesis of A. fumigatus.



Construction and pathogenicity of Aspergillus fumigatus mutants that do not produce the ribotoxin restrictocin

It is demonstrated that restrictocin is not an important virulence factor in this model of IPA and histological examination of infectediung tissue did not reveal any obvious differences in the number or size of fungal colonies or in the polymorphonuclearleucocyte response.

Regulation of fungal extracellular proteases and their role in mammalian pathogenesis

The involvement of wide-domain regulators in the control of protease production is discussed and an example of how one of these regulators (encoded by the areA gene) has been used in virul...

Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph.

The induction and repression of nitrate reductase in the fungus Aspergillus nidulans.

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  • Biology
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Mutational analysis reveals dispensability of the N‐terminal region of the Aspergillus transcription factor mediating nitrogen metabolite repression

Mutational analysis has enabled identification and localization of an upstream exon of the areA gene of Aspergillus nidulans mediating nitrogen metabolite repression and suppression of frameshift mutations by compensating mutations preventing intron splicing suggests that insertion of a markedly hydrophobic sequence can impair AREA function.

The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger.

Results show that the regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans reverts to a partly reciprocal phenotype by replacing the mutant valine by methionine, and also identifies sequence changes associated with specificity mutations.

nit-2, the major nitrogen regulatory gene of Neurospora crassa, encodes a protein with a putative zinc finger DNA-binding domain

The nitrogen regulatory circuit of Neurospora crassa consists of a set of unlinked structural genes which specify various nitrogen catabolic enzymes plus control genes and metabolic effectors which

Molecular Cloning: A Laboratory Manual

The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.

Virulence of Aspergillus fumigatus double mutants lacking restriction and an alkaline protease in a low-dose model of invasive pulmonary aspergillosis

It is concluded that Alp and restrictocin are not important virulence determinants in pulmonary infection.

The genus Aspergillus