The role of protamine amount in the transfection performance of cationic SLN designed as a gene nanocarrier.

  title={The role of protamine amount in the transfection performance of cationic SLN designed as a gene nanocarrier.},
  author={Eleonora Vighi and Monica Montanari and Barbara Ruozi and Valentina Iannuccelli and Eliana Leo},
  journal={Drug delivery},
  volume={19 1},
Cationic solid lipid nanoparticles (SLN) have been recently proposed as non-viral vectors in systemic gene therapy. The aim of this study was to evaluate the effect of the protamine amount used as the transfection promoter in SLN-mediated gene delivery. Three protamine-SLN samples (Pro25, Pro100, and Pro200) prepared by adding increasing amounts of protamine were characterized for their size, zeta potential, and protamine loading level. The samples were evaluated for pDNA complexation ability… CONTINUE READING


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Publications referenced by this paper.
Showing 1-10 of 30 references

Characterization of Protamine as a tranfection accelerator for gene delivery

Y. Tsuchiya, T. Ishti, Y. Okahata, T. Sato
J Bioact Comp Polym • 2006
View 4 Excerpts
Highly Influenced

Packaging paternal chromosomes with protamine

Nature Genetics • 2001
View 11 Excerpts
Highly Influenced

Fatty acid transport and metabolism in HepG2 cells.

American journal of physiology. Gastrointestinal and liver physiology • 2006
View 13 Excerpts
Highly Influenced

Nuclear localization of cationic solid lipid nanoparticles containing Protamine as transfection promoter.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V • 2010
View 3 Excerpts

Short- and long-term stability study of lyophilized solid lipid nanoparticles for gene therapy

A. Del Pozo-Rodríguez, M. A. Solinís, A. R. Gascón, Pedraz, J.L
Eur J Pharm Biopharm • 2009
View 1 Excerpt

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