The role of protamine amount in the transfection performance of cationic SLN designed as a gene nanocarrier.

@article{Vighi2012TheRO,
  title={The role of protamine amount in the transfection performance of cationic SLN designed as a gene nanocarrier.},
  author={Eleonora Vighi and Monica Montanari and Barbara Ruozi and Valentina Iannuccelli and Eliana Leo},
  journal={Drug delivery},
  year={2012},
  volume={19 1},
  pages={1-10}
}
Cationic solid lipid nanoparticles (SLN) have been recently proposed as non-viral vectors in systemic gene therapy. The aim of this study was to evaluate the effect of the protamine amount used as the transfection promoter in SLN-mediated gene delivery. Three protamine-SLN samples (Pro25, Pro100, and Pro200) prepared by adding increasing amounts of protamine were characterized for their size, zeta potential, and protamine loading level. The samples were evaluated for pDNA complexation ability… CONTINUE READING

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