The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases.

@article{Ribeiro2004TheRO,
  title={The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases.},
  author={Sofia C. Ribeiro and Gabriel Ant{\'o}nio Amaro Monteiro and Duarte M. F. Prazeres},
  journal={The journal of gene medicine},
  year={2004},
  volume={6 5},
  pages={565-73}
}
BACKGROUND Nuclease degradation of plasmid DNA (pDNA) vectors after delivery and during trafficking to the nucleus is a barrier to gene expression. This barrier may be circumvented by shielding the pDNA from the nuclease-rich cell environment with adjuvants or by using nuclease inhibitors. A different alternative that is explored in this work is to make pDNA vectors more nuclease-resistant a priori. METHODS AND RESULTS The hypothesis that a significant part of nuclease attack is directed… CONTINUE READING