The role of nucleoid‐associated proteins in the organization and compaction of bacterial chromatin

  title={The role of nucleoid‐associated proteins in the organization and compaction of bacterial chromatin},
  author={Remus T. Dame},
  journal={Molecular Microbiology},
  • R. T. Dame
  • Published 1 May 2005
  • Biology, Physics, Chemistry
  • Molecular Microbiology
The bacterial chromosomal DNA is folded into a compact structure called nucleoid. The shape and size of this ‘body’ is determined by a number of factors. Major players are DNA supercoiling, macromolecular crowding and architectural proteins, associated with the nucleoid, which are the topic of this MicroReview. Although many of these proteins were identified more than 25 years ago, the molecular mechanisms involved in the organization and compaction of DNA have only started to become clear in… 
The bacterial nucleoid: A highly organized and dynamic structure
It emerged that multiple mechanisms cooperate to establish a dynamic assembly of supercoiled domains, which are stacked in consecutive order to adopt a defined higher‐level organization.
Impact of self-association on the architectural properties of bacterial nucleoid proteins.
Nucleoid-associated proteins in Crenarchaea.
The knowledge currently available on architectural proteins in Crenarchaea is summarized and the crenarchaeal nucleoid shows similarities with that of Bacteria.
Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome
The results show that the distribution of these proteins is biased to intergenic parts of the genome, and that the binding profiles overlap, so some targets are associated with combinations of bound FIS, H-NS and IHF.
Structure and dynamics of the crenarchaeal nucleoid.
The molecular properties of cren archaeal chromatin proteins are described and the possible role of these architectural proteins in organizing the crenarchaeal Chromatin and in gene regulation is discussed.
The Major Architects of Chromatin: Architectural Proteins in Bacteria, Archaea and Eukaryotes
It is concluded that the underlying mechanisms that shape and remodel genomes are remarkably similar among bacteria, archaea and eukaryotes.
Organization and segregation of bacterial chromosomes
It is argued that the key feature of compaction is the orderly folding of DNA along adjacent segments and that this organization provides easy and efficient access for protein–DNA transactions and has a central role in driving segregation.


Structure and partitioning of bacterial DNA: determined by a balance of compaction and expansion forces?
Two hypotheses are discussed: (i) the structure of the Escherichia coli nucleoid is determined by DNA binding proteins and DNA supercoiling, representing a compaction force on the one hand, and by the coupled transcription/translation/translocation of plasma membrane and cell wall proteins, representing an expansion force.
An architectural role of the Escherichia coli chromatin protein FIS in organising DNA.
It is proposed, in addition to the previously inferred stabilisation of tightly bent DNA microloops in the upstream regions of certain promoters, that FIS may perform the distinct architectural function of organising branched plectonemes in the E.coli nucleoid.
H-NS mediated compaction of DNA visualised by atomic force microscopy.
A model for global condensation of the chromosomal DNA by H-NS is proposed and large globular structures were identified that incorporated a considerable amount of DNA.
Dual architectural roles of HU: formation of flexible hinges and rigid filaments.
This study used magnetic tweezers and atomic force microscopy to quantify HU-induced DNA bending and condensation and revealed that HU can have two opposing mechanical effects depending on the protein concentration.
Osmotic compaction of supercoiled DNA into a bacterial nucleoid.
  • T. Odijk
  • Physics, Biology
    Biophysical chemistry
  • 1998
Macromolecular crowding and the mandatory condensation of DNA in bacteria
The role of co‐transcriptional translation and protein translocation (transertion) in bacterial chromosome segregation
It is proposed that the random diffusion of DNA supercoil segments is transiently constrained by the process of co‐ transcriptional translation and translocation (transertion) of membrane proteins.
IHF and HU: flexible architects of bent DNA.