The role of nucleoid‐associated proteins in the organization and compaction of bacterial chromatin

@article{Dame2005TheRO,
  title={The role of nucleoid‐associated proteins in the organization and compaction of bacterial chromatin},
  author={Remus T. Dame},
  journal={Molecular Microbiology},
  year={2005},
  volume={56}
}
  • R. T. Dame
  • Published 1 May 2005
  • Biology, Physics, Chemistry
  • Molecular Microbiology
The bacterial chromosomal DNA is folded into a compact structure called nucleoid. The shape and size of this ‘body’ is determined by a number of factors. Major players are DNA supercoiling, macromolecular crowding and architectural proteins, associated with the nucleoid, which are the topic of this MicroReview. Although many of these proteins were identified more than 25 years ago, the molecular mechanisms involved in the organization and compaction of DNA have only started to become clear in… 
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It emerged that multiple mechanisms cooperate to establish a dynamic assembly of supercoiled domains, which are stacked in consecutive order to adopt a defined higher‐level organization.
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Nucleoid-associated proteins in Crenarchaea.
TLDR
The knowledge currently available on architectural proteins in Crenarchaea is summarized and the crenarchaeal nucleoid shows similarities with that of Bacteria.
Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome
TLDR
The results show that the distribution of these proteins is biased to intergenic parts of the genome, and that the binding profiles overlap, so some targets are associated with combinations of bound FIS, H-NS and IHF.
Structure and dynamics of the crenarchaeal nucleoid.
TLDR
The molecular properties of cren archaeal chromatin proteins are described and the possible role of these architectural proteins in organizing the crenarchaeal Chromatin and in gene regulation is discussed.
The Major Architects of Chromatin: Architectural Proteins in Bacteria, Archaea and Eukaryotes
TLDR
It is concluded that the underlying mechanisms that shape and remodel genomes are remarkably similar among bacteria, archaea and eukaryotes.
Organization and segregation of bacterial chromosomes
TLDR
It is argued that the key feature of compaction is the orderly folding of DNA along adjacent segments and that this organization provides easy and efficient access for protein–DNA transactions and has a central role in driving segregation.
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