The role of metals in catalysis by the restriction endonuclease Bam HI

@article{Viadiu1998TheRO,
  title={The role of metals in catalysis by the restriction endonuclease Bam HI},
  author={Hector Viadiu and Aneel K. Aggarwal},
  journal={Nature Structural Biology},
  year={1998},
  volume={5},
  pages={910-916}
}
Type II restriction enzymes are characterized by their remarkable specificity and simplicity. They require only divalent metals (such as Mg2+ or Mn2+) as cofactors to catalyze the hydrolysis of DNA. However, most of the structural work on endonucleases has been performed in the absence of metals, leaving unanswered questions about their mechanisms of DNA cleavage. Here we report structures of the endonuclease BamHI–DNA complex, determined in the presence of Mn2+ and Ca2+, that describe the… Expand
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