The role of inter-subunit ionic interactions in the assembly of Physalis mottle tymovirus

  title={The role of inter-subunit ionic interactions in the assembly of Physalis mottle tymovirus},
  author={Mahadevaiah Umashankar and M. R. V. Murthy and S. Anil Singh and A. G. Appu Rao and Handanahal Subbarao Savithri},
  journal={Archives of Virology},
Summary.Physalis mottle tymovirus (PhMV) is a small spherical plant virus with its RNA genome encapsidated in a protein shell made of 180 identical coat protein (CP) subunits. The amino acid residues involved in two interfacial salt bridges, Asp-83/Arg-159 and Arg-68/Asp-150 and Lys-153, were targeted for mutagenesis with a view to delineate the role of interfacial ionic interactions in the subunit folding and assembly of the virus. R159A and D83A-R159A recombinant CP (rCP) mutants formed… 
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