The role of hydration in enzyme activity and stability: 1. Water adsorption by alcohol dehydrogenase in a continuous gas phase reactor.

@article{Yang1996TheRO,
  title={The role of hydration in enzyme activity and stability: 1. Water adsorption by alcohol dehydrogenase in a continuous gas phase reactor.},
  author={F Yang and Alan J. Russell},
  journal={Biotechnology and bioengineering},
  year={1996},
  volume={49 6},
  pages={700-8}
}
The adsorption of water by alcohol dehydrogenase from baker's yeast (YADH) has been measured in a continuous-flow gas reactor at varying temperatures. Adsorption isotherms in the presence of gaseous organic substrates are compared to those from organic-free gas mixtures. Almost no effect of the hydrophobic molecule on total water adsorption was observed. A rarely mentioned multilayer isotherm model from the 1930s, the Huttig's isotherm, has been found to fit the experimental data with extremely… CONTINUE READING