The role of glyoxalase I in the detoxification of methylglyoxal and in the activation of the KefB K+ efflux system in Escherichia coli.

@article{MacLean1998TheRO,
  title={The role of glyoxalase I in the detoxification of methylglyoxal and in the activation of the KefB K+ efflux system in Escherichia coli.},
  author={Morag J MacLean and L. S. Ness and Gail P. Ferguson and Ian Rylance Booth},
  journal={Molecular microbiology},
  year={1998},
  volume={27 3},
  pages={
          563-71
        }
}
The glyoxalase I gene (gloA) of Escherichia coli has been cloned and used to create a null mutant. Cells overexpressing glyoxalase I exhibit enhanced tolerance of methylglyoxal (MG) and exhibit elevated rates of detoxification, although the increase is not stoichiometric with the change in enzyme activity. Potassium efflux via KefB is also enhanced in the overexpressing strain. Analysis of the physiology of the mutant has revealed that growth and viability are quite normal, unless the cell is… CONTINUE READING

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