The role of eucaryotic factor Tu in protein synthesis. The measurement of the elongation factor Tu content of rabbit reticulocytes and other mammalian cells by a sensitive radioimmunoassay.

  • L. I. Slobin
  • Published 1980 in European journal of biochemistry

Abstract

A sensitive radioimmunoassay for eucaryotic elongation factor Tu (eEF-TU) was developed using radioiodinated elongation factor T (eEF-T) and goat anti-(rabbit eEF-T) immunoglobulins coupled to a solid support. eEF-T was iodinated with 125I to a specific activity of 7 x 10(3) counts min-1 ng-1 using a system employing lactoperoxidase and glucose oxidase… (More)

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