The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes

@inproceedings{Banfi2007TheRO,
  title={The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes},
  author={Giuseppe Banfi and Gian Luca Salvagno and Giuseppe Lippi},
  booktitle={Clinical Chemistry and Laboratory Medicine},
  year={2007}
}
Abstract Anticoagulants are used to prevent clot formation both in vitro and in vivo. In the specific field of in vitro diagnostics, anticoagulants are commonly added to collection tubes either to maintain blood in the fluid state for hematological testing or to obtain suitable plasma for coagulation and clinical chemistry analyses. Unfortunately, no universal anticoagulant that could be used for evaluation of several laboratory parameters in a sample from a single test tube is available so far… 

Figures and Tables from this paper

Effect of Different Anticoagulant Agents on Immune-Related Genes in Leukocytes Isolated from the Whole-Blood of Holstein Cows

Anticoagulants, such as ethylenediaminetetraacetic acid (EDTA), sodium citrate (Na-citrate), or heparin are normally used in hematological clinical tests to prevent coagulation. Although

Contamination of lithium heparin blood by K2-ethylenediaminetetraacetic acid (EDTA): an experimental evaluation

The concentration of calcium, magnesium, potassium, chloride and LD appears to be dramatically biased by even modest K2 EDTA contamination, whereas ALT, bilirubin, creatinine and lipase appear overall less vulnerable towards K2EDTA contamination.

Control of Oral Anticoagulant Therapy Using EDTA Plasma

Despite the very good correlation observed between results of citrate and EdTA samples, no clinical agreement was established between the two methods and the use of EDTA samples for performing PT tests by manual Quick method is not acceptable.

Measurement of iron in serum and EDTA plasma for screening of blood transfusion in sports.

concluding that the measurement of iron in ethylenediamine tetraacetic acid (EDTA) plasma may be used as a simple and cost effective approach for screening blood transfusion misuse in sports.

Influence of Anticoagulants on Determination of H2O2 Levels in Blood: Comparison of Citrate and EDTA

Evidence is presented to advance that EDTA anticoagulant is unsuitable for laboratory testing of H2O2 in plasma, and the test results support the suggestion that ratio of EDTA to iron is greater than 1:1.

Magnesium Sulfate as an Alternative In Vitro Anticoagulant for the Measurement of Platelet Parameters?

The aim of this study was to evaluate MgSO4 as an in vitro anticoagulant for platelet count, MPV, platelet distribution width, and platelet activation and it revealed similar results when measured by the fluorescent optical method.

Stability of thromboxane in blood samples

Investigating if platelets continue to form thromboxane A2 in the blood tube after sample collection finds storage conditions, type of sampling vial and time from sampling until sample processing (centrifuging) has a major impact on throm boxane B2 stability.

Pseudothrombocytopenia induced by incubation at 37 ℃ in ethylenediaminetetraacetic acid tubes

Ethylenediaminetetraacetic acid (EDTA) is the anticoagulant currently used for cell blood counts. Several studies showed that exposure of platelets (PLTs) to EDTA for a short period of time promote

Effective estimation of correct platelet counts in pseudothrombocytopenia using an alternative anticoagulant based on magnesium salt

It is concluded that in patients with known or suspected pseudothrombocytopenia the magnesium‐anticoagulant blood samples may be recommended for platelet counting.

Appropriate Handling, Processing and Analysis of Blood Samples Is Essential to Avoid Oxidation of Vitamin C to Dehydroascorbic Acid

T careful handling and processing of samples, combined with appropriate analysis, is crucial for accurate determination of ascorbate and DHA in clinical samples.
...

References

SHOWING 1-10 OF 139 REFERENCES

Effects of anticoagulants in amino acid analysis: comparisons of heparin, EDTA, and sodium citrate in vacutainer tubes for plasma preparation.

The analytic variation among samples treated with heparin and EDTA are investigated, and the use of different anticoagulants for plasma sample preparation can increase the potential for analytic errors.

Recommendations of the International Council for Standardization in Haematology for Ethylenediaminetetraacetic Acid Anticoagulation of Blood for Blood Cell Counting and Sizing. International Council for Standardization in Haematology: Expert Panel on Cytometry.

Dipotassium EDTA is recommended as the anticoagulant of choice in specimen collection for blood cell counting and sizing and has been shown to affect the red blood cell size more at increased concentrations and on storage than the dipotassium salt.

Use of EDTA samples for prothrombin time measurement in patients receiving oral anticoagulants.

A good correlation was observed between INR results with citrate and EDTA samples from patients receiving oral anticoagulants using Owren's PT reagent with the same citrate calibration.

Evaluation of the anticoagulants EDTA and citrate, theophylline, adenosine, and dipyridamole (CTAD) for assessing platelet activation on the ADVIA 120 hematology system.

When determining plateletactivation ex vivo on the ADVIA 120, blood should be collected into E/C, stored at 4 degrees C, and analyzed between 60 and 180 min later; these conditions ensure maximum platelet sphering without concurrent artifactual platelet activation.

Variations in blood levels of aminoglycosides related to in vitro anticoagulant usage.

The anticoagulant used was of critical importance in determining AMG blood levels, which were underestimated when citrate or heparin were present, and the higher the level of AMG in serum, the greater the discrepancies between drug concentrations measured with different antICOagulants.

K2- or K3-EDTA: the anticoagulant of choice in routine haematology?

It is concluded that the choice of anticoagulant, its use at an appropriate concentration and the age of the blood sample are important matters and should be given due consideration.

Effects of contamination of blood specimens with liquid potassium-EDTA anticoagulant

The identification of subtle contamination of blood specimens for biochemical analysis requires a sensitive method for measurement of EDTA itself.

DX-9065a, a specific factor Xa inhibitor, as a universal anticoagulant for blood collection tubes.

...