The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes

@inproceedings{Banfi2007TheRO,
  title={The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes},
  author={Giuseppe Banfi and Gian Luca Salvagno and Giuseppe Lippi},
  booktitle={Clinical chemistry and laboratory medicine},
  year={2007}
}
Abstract Anticoagulants are used to prevent clot formation both in vitro and in vivo. In the specific field of in vitro diagnostics, anticoagulants are commonly added to collection tubes either to maintain blood in the fluid state for hematological testing or to obtain suitable plasma for coagulation and clinical chemistry analyses. Unfortunately, no universal anticoagulant that could be used for evaluation of several laboratory parameters in a sample from a single test tube is available so far… Expand
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166 Citations

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Contamination of lithium heparin blood by K2-ethylenediaminetetraacetic acid (EDTA): an experimental evaluation
TLDR
The concentration of calcium, magnesium, potassium, chloride and LD appears to be dramatically biased by even modest K2 EDTA contamination, whereas ALT, bilirubin, creatinine and lipase appear overall less vulnerable towards K2EDTA contamination. Expand
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Despite the very good correlation observed between results of citrate and EdTA samples, no clinical agreement was established between the two methods and the use of EDTA samples for performing PT tests by manual Quick method is not acceptable. Expand
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Background: H2O2 is supposed to be part of the laboratory test panel for assessment of oxidative stress. This molecule contributes to oxidative stress by reacting with Fe2+ ions to produce hydroxyl… Expand
Magnesium Sulfate as an Alternative In Vitro Anticoagulant for the Measurement of Platelet Parameters?
TLDR
The aim of this study was to evaluate MgSO4 as an in vitro anticoagulant for platelet count, MPV, platelet distribution width, and platelet activation and it revealed similar results when measured by the fluorescent optical method. Expand
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TLDR
Investigating if platelets continue to form thromboxane A2 in the blood tube after sample collection finds storage conditions, type of sampling vial and time from sampling until sample processing (centrifuging) has a major impact on throm boxane B2 stability. Expand
Pseudothrombocytopenia induced by incubation at 37 ℃ in ethylenediaminetetraacetic acid tubes
Ethylenediaminetetraacetic acid (EDTA) is the anticoagulant currently used for cell blood counts. Several studies showed that exposure of platelets (PLTs) to EDTA for a short period of time promote… Expand
Effective estimation of correct platelet counts in pseudothrombocytopenia using an alternative anticoagulant based on magnesium salt
TLDR
It is concluded that in patients with known or suspected pseudothrombocytopenia the magnesium‐anticoagulant blood samples may be recommended for platelet counting. Expand
Appropriate Handling, Processing and Analysis of Blood Samples Is Essential to Avoid Oxidation of Vitamin C to Dehydroascorbic Acid
TLDR
T careful handling and processing of samples, combined with appropriate analysis, is crucial for accurate determination of ascorbate and DHA in clinical samples. Expand
Addition of protease inhibitors to EDTA containing blood collection tubes does not deliver significant advantage for preservation of plasma for down-stream analysis
TLDR
This study tested the proteome and peptidome stability of matched plasma samples collected from healthy individuals, and results obtained demonstrated a lower number of endogenous peptides suggesting preservation of some proteins in plasma treated with the protease inhibitor cocktail. Expand
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