The role of cluster formation and metastable liquid - Liquid phase separation in protein crystallization

@article{Zhang2012TheRO,
  title={The role of cluster formation and metastable liquid - Liquid phase separation in protein crystallization},
  author={F. Zhang and F. Roosen-Runge and A. Sauter and R. Roth and M. Skoda and R. Jacobs and M. Sztucki and F. Schreiber},
  journal={Faraday Discussions},
  year={2012},
  volume={159},
  pages={313-325}
}
We discuss the phase behavior and in particular crystallization of a model globular protein (beta-lactoglobulin) in solution in the presence of multivalent electrolytes. It has been shown previously that negatively charged globular proteins at neutral pH in the presence of multivalent counterions undergo a “re-entrant condensation (RC)” phase behavior (Zhang et al., Phys. Rev. Lett., 2008, 101, 148101), i.e. a phase-separated regime occurs in between two critical salt concentrations, c* < c… Expand
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