The role of cellulase concentration in determining the degree of synergism in the hydrolysis of microcrystalline cellulose.

@article{Woodward1988TheRO,
  title={The role of cellulase concentration in determining the degree of synergism in the hydrolysis of microcrystalline cellulose.},
  author={Jonathan D Woodward and Marcos Paulo Antunes de Lima and Norman E. Lee},
  journal={The Biochemical journal},
  year={1988},
  volume={255 3},
  pages={895-9}
}
Microcrystalline cellulose (10 mg of Avicel/ml) was hydrolysed to glucose by different concentrations of the purified cellulase components endoglucanase (EG) II and cellobiohydrolases (CBH) I and II, alone and in combination with each other, in the presence of excess beta-glucosidase. At a concentration of 360 micrograms/ml (160 micrograms of EG II/ml, 100 micrograms of CBH I/ml and 100 micrograms of CBH II/ml) the degree of synergism among them was negligible. As the concentration of cellulase… CONTINUE READING

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