The role of carbohydrate in recombinant human erythropoietin.

  title={The role of carbohydrate in recombinant human erythropoietin.},
  author={Eisuke Tsuda and Gosei Kawanishi and Masamichi Ueda and Satohiro Masuda and Ryuzo Sasaki},
  journal={European journal of biochemistry},
  volume={188 2},
1. Recombinant human erythropoietin has N-linked sugar [Tsuda et al., (1988) Biochemistry 27, 5646-5654]. Here we have demonstrated the presence of O-linked sugar (0.85 mol/mol erythropoietin) composed of sialic acid and Gal beta(1-3)GalNAc. 2. To investigate the role of these sugars, erythropoietins deglycosylated to different extents were prepared using… CONTINUE READING