The role of budABC on 1,3-propanediol and 2,3-butanediol production from glycerol in Klebsiella pneumoniae CICIM B0057

  title={The role of budABC on 1,3-propanediol and 2,3-butanediol production from glycerol in Klebsiella pneumoniae CICIM B0057},
  author={Xinyao Lu and Guangjian Ji and H. Zong and B. Zhuge},
  pages={439 - 444}
ABSTRACT 1,3-propanediol (1,3-PD) is an important compound from which many others can be synthesized. 2,3-butanediol (BDO) is the key by-product in the biosynthesis of 1,3-PD from glycerol, but it impedes its downstream purification. In Klebsiella, the budA, budB and budC genes encode enzymes that are responsible for the synthesis of BDO. In this study, 3 individual antisense RNAs were designed to repress the expression and hence activity of BudA-C. Compared with the parent strains, the… Expand
Reprogramming the metabolism of Klebsiella pneumoniae for efficient 1,3-propanediol production
Abstract The production of 1,3-propanediol (1,3-PD) from glycerol by Klebsiella pneumoniae is limited by synthesis of numerous byproducts. Among them, the accumulation of acetate has the largestExpand
Production of polyhydroxybutyrate (PHB) by a novel Klebsiella pneumoniae strain using low-cost media from fruit peel residues
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Regulatory non-coding sRNAs in bacterial metabolic pathway engineering.
The design, characterization, and impact of ncRNAs in engineering both native and exogenous metabolic pathways in bacteria are reviewed and the opportunities afforded by recent high-throughput approaches for characterizing sRNA regulators and their corresponding networks are considered to showcase their potential applications and impact in engineering bacterial metabolism. Expand


Observation of 2,3-butanediol biosynthesis in Lys regulator mutated Klebsiella pneumoniae at gene transcription level.
This is by far the first observation of 2,3-BDO regulation mechanism at gene transcription level in K. pneumoniae, and therefore may be useful for understanding and improving 2, 3- BDO biosynthesis metabolic network. Expand
Expression of 1,3-propanediol oxidoreductase and its isoenzyme in Klebsiella pneumoniae for bioconversion of glycerol into 1,3-propanediol
Experimental results showed that the peak values for 3-HPA production in broth of the three recombinant strains were less than 25% of that of the parent strain, which has important implications for further studies involving use of YqhD and DhaT for bioconversion of glycerol into 1,3-PD. Expand
budC knockout in Klebsiella pneumoniae for bioconversion from glycerol to 1,3‐propanediol
The results of the mutant fed‐batch fermentation showed that the 1,3‐PDO concentration, productivity per cell dry weight, and conversion rate showed increasing by 10%, 15%, and 11% compared with the parent strain, which implies that K. pneumoniae possesses a pathway of the 2, 3‐BD cycle as a replenishment pathway. Expand
Engineering Klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene
The results suggested that inactivation of aldA led to a significantly improved 2,3-BD production. Expand
NADH plays the vital role for chiral pure D-(-)-2,3-butanediol production in Bacillus subtilis under limited oxygen conditions.
The strategy for increasing the pool of NADH availability, the key factor for 2,3-butanediol production, was designed through low dissolved oxygen control, adding reducing substrates and rationally metabolic engineering, and a transhydrogenase encoded by udhA was introduced to provide more NADH from NADPH and allowed enhanced 2, 3-butane production. Expand
Characterization of the genes of the 2,3-butanediol operons from Klebsiella terrigena and Enterobacter aerogenes
The genes involved in the 2,3-butanediol pathway coding for alpha-acetolactate decarboxylase, alpha-acetolactate synthase (alpha-ALS), and acetoin (diacetyl) reductase were isolated from KlebsiellaExpand
The Bacillus subtilis ydjL (bdhA) Gene Encodes Acetoin Reductase/2,3-Butanediol Dehydrogenase
  • W. Nicholson
  • Medicine, Biology
  • Applied and Environmental Microbiology
  • 2008
From the data, it is proposed that the major AR/BDH-encoding gene ydjL be renamed bdhA, suggesting the existence of a second gene encoding a minor AR activity. Expand
Integrated Regulation of Acetoin Fermentation by Quorum Sensing and pH in Serratia plymuthica RVH1
Interestingly, however, it was observed that induction of budR transcription by OHHL or acetate did not require BudR, indicating the involvement of additional regulatory factors in relaying these environmental signals to the budR promoter. Expand
Glycerol Dehydrogenase Plays a Dual Role in Glycerol Metabolism and 2,3-Butanediol Formation in Klebsiella pneumoniae *
Whereas DhaD is specialized for glycerol utilization, GldA plays a role in backup compensation and can turn into a more proficient catalyst to promote a survival advantage to the organism, suggesting a mechanism involved in evolution of the enzyme. Expand
Present state and perspective of downstream processing of biologically produced 1,3-propanediol and 2,3-butanediol
It is argued that separation technologies such as aqueous two-phase extraction with short chain alcohols, pervaporation, reverse osmosis, and in situ extractive or pervaporative fermentations deserve more attention in the future. Expand