The role of aromatic and acidic amino acids in the electron transfer reaction catalyzed by spinach ferredoxin-dependent glutamate synthase.

@article{Hirasawa1998TheRO,
  title={The role of aromatic and acidic amino acids in the electron transfer reaction catalyzed by spinach ferredoxin-dependent glutamate synthase.},
  author={Masakazu Hirasawa and John K Hurley and Zdzislaw Salamon and Gordon Tollin and John L. Markley and Heung-Chin Cheng and Bingle Xia and David B. Knaff},
  journal={Biochimica et biophysica acta},
  year={1998},
  volume={1363 2},
  pages={134-46}
}
Treatment of the ferredoxin-dependent, spinach glutamate synthase with N-bromosuccinimide (NBS) modifies 2 mol of tryptophan residues per mol of enzyme, without detectable modification of other amino acids, and inhibits enzyme activity by 85% with either reduced ferredoxin or reduced methyl viologen serving as the source of electrons. The inhibition of ferredoxin-dependent activity resulting from NBS treatment arises entirely from a decrease in the turnover number. Complex formation of… CONTINUE READING