The role of SmpB protein in trans-translation.

@article{Shimizu2002TheRO,
  title={The role of SmpB protein in trans-translation.},
  author={Yoshihiro Shimizu and Takuya Ueda},
  journal={FEBS letters},
  year={2002},
  volume={514 1},
  pages={
          74-7
        }
}
The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto… CONTINUE READING
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