The role of IRES trans-acting factors in regulating translation initiation.

@article{King2010TheRO,
  title={The role of IRES trans-acting factors in regulating translation initiation.},
  author={Helen A King and Laura C. Cobbold and Anne E. Willis},
  journal={Biochemical Society transactions},
  year={2010},
  volume={38 6},
  pages={
          1581-6
        }
}
The majority of mRNAs in eukaryotic cells are translated via a method that is dependent upon the recognition of, and binding to, the methylguanosine cap at the 5' end of the mRNA, by a set of protein factors termed eIFs (eukaryotic initiation factors). However, many of the eIFs involved in this process are modified and become less active under a number of pathophysiological stress conditions, including amino acid starvation, heat shock, hypoxia and apoptosis. During these conditions, the… 
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This review will clearly explain the required experiments, and the pros and cons of various assays, used to determine whether (or not) an RNA element functions as an IRES to promote initiation of translation and hopes that demystifying the accepted methods for assaying IRES activity will open the study of this important mechanism to the broader community.
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TLDR
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