The role of GlcNAc-PI-de-N-acetylase gene by gene knockout through homologous recombination and its consequences on survival, growth and infectivity of Leishmania major in in vitro and in vivo conditions.

@article{Almani2016TheRO,
  title={The role of GlcNAc-PI-de-N-acetylase gene by gene knockout through homologous recombination and its consequences on survival, growth and infectivity of Leishmania major in in vitro and in vivo conditions.},
  author={Pooya Ghasemi Nejad Almani and Iraj Sharifi and Bahram Kazemi and Zahra Babaei and Mojgan Bandehpour and S A Nejat Salari and Ebrahim Saedi Dezaki and Farideh Tohidi and Mohammad Ali Mohammadi},
  journal={Acta tropica},
  year={2016},
  volume={154},
  pages={
          63-72
        }
}
At present, there are no efficacious vaccines or effective drugs against leishmaniasis; therefore new and innovative control methods are urgently required. One way to achieve this important goal is through using reverse genetic engineering to evaluate important enzymes, proteins and macromolecules. One of the most important enzymes for Glycosylphosphatidylinositol (GPI) biosynthetic pathways is GlcNAc-PI-de-N-acetylase (GPI12). The molecular constructs were cloned in Escherichia coli strain Top… CONTINUE READING
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