The role of DNA polymerase activity in human non-homologous end joining.

@article{Pospiech2001TheRO,
  title={The role of DNA polymerase activity in human non-homologous end joining.},
  author={Helmut Pospiech and Anna K Rytk{\"o}nen and Juhani E. Syv{\"a}oja},
  journal={Nucleic acids research},
  year={2001},
  volume={29 15},
  pages={3277-88}
}
In mammalian cells, DNA double-strand breaks are repaired mainly by non-homologous end joining, which modifies and ligates two DNA ends without requiring extensive base pairing interactions for alignment. We investigated the role of DNA polymerases in DNA-PK-dependent end joining of restriction-digested plasmids in vitro and in vivo. Rejoining of DNA blunt ends as well as those with partially complementary 5' or 3' overhangs was stimulated by 20-53% in HeLa cell-free extracts when dNTPs were… CONTINUE READING