The rhodopsin-transducin complex houses two distinct rhodopsin molecules.

@article{Jastrzebska2013TheRC,
  title={The rhodopsin-transducin complex houses two distinct rhodopsin molecules.},
  author={Beata Jastrzebska and Philippe Ringler and Krzysztof Palczewski and Andreas Engel},
  journal={Journal of structural biology},
  year={2013},
  volume={182 2},
  pages={164-72}
}
Upon illumination the visual receptor rhodopsin (Rho) transitions to the activated form Rho(∗), which binds the heterotrimeric G protein, transducin (Gt) causing GDP to GTP exchange and Gt dissociation. Using succinylated concanavalin A (sConA) as a probe, we visualized native Rho dimers solubilized in 1mM n-dodecyl-β-d-maltoside (DDM) and Rho monomers in 5mM DDM. By nucleotide depletion and affinity chromatography together with crosslinking and size exclusion chromatography, we trapped and… CONTINUE READING

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Asymmetry of the rhodopsin dimer in complex with transducin.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology • 2013

Distinct roles of metabotropic glutamate receptor dimerization in agonist activation and Gprotein coupling

D. El Moustaine, S. Granier, +7 authors J. P. Pin
Proc. Natl. Acad. Sci. USA • 2012

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