The retinoblastoma gene product regulates progression through the G1 phase of the cell cycle

@article{Goodrich1991TheRG,
  title={The retinoblastoma gene product regulates progression through the G1 phase of the cell cycle},
  author={David W. Goodrich and Nan Wang and Yue-wei Qian and Eva Y.-H. P. Lee and Wen-Hwa Lee},
  journal={Cell},
  year={1991},
  volume={67},
  pages={293-302}
}
The RB gene product is a nuclear phosphoprotein that undergoes cell cycle-dependent changes in its phosphorylation status. To test whether RB regulates cell cycle progression, purified RB proteins, either full-length or a truncated form containing the T antigen-binding region, were injected into cells. Injection of either protein early in G1 inhibits progression into S phase. Co-injection of anti-RB antibodies antagonizes this effect. Injection of RB into cells arrested at G1/S or late in G1… Expand
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The cell cycle-dependent phosphorylation of Rb is demonstrated and a model to explain how Rb may suppress cell growth by acting as a cell cycle regulatory element is proposed. Expand
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