The requirement for the basal transcription factor IIE is determined by the helical stability of promoter DNA.

@article{Holstege1995TheRF,
  title={The requirement for the basal transcription factor IIE is determined by the helical stability of promoter DNA.},
  author={Frank Cp Holstege and Dean Tantin and Michael Carey and Peter C. van der Vliet and H. Th. Marc Timmers},
  journal={The EMBO journal},
  year={1995},
  volume={14 4},
  pages={810-9}
}
The role of the basal transcription factor TFIIE was investigated in RNA polymerase II transcription reactions reconstituted with purified proteins. Using negatively supercoiled templates, which circumvent the requirement for TFIIH, we observed that transcription from the adenovirus major-late (ML) core promoter is more dependent on TFIIE than transcription from the adenovirus E4 (E4) or mouse mammary tumor virus (MMTV) promoters. For all three promoters, an increase in the ionic strength of… CONTINUE READING

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