The removal of the type-2 copper from Rhus vernicifera laccase.

@article{Li1992TheRO,
  title={The removal of the type-2 copper from Rhus vernicifera laccase.},
  author={Ji-liang Li and David R McMillin},
  journal={Biochimica et biophysica acta},
  year={1992},
  volume={1160 3},
  pages={239-45}
}
We have studied the removal of the type-2 copper from tree laccase (Rhus vernicifera) by treatment with EDTA at pH 5.2 in the presence of a redox buffer containing ferri- and ferrocyanide. The efficiency with which the copper is removed depends on the Fe(CN) 6(4-)/Fe(CN) 6(3-) ratio. We have varied this ratio from approx. 2:1 to about 50:1 and the best… CONTINUE READING