The release of heparinase from the periplasmic space of flavobacterium heparinum by three-step osmotic shock

@article{ZimmermannJosephJ1991TheRO,
  title={The release of heparinase from the periplasmic space of flavobacterium heparinum by three-step osmotic shock},
  author={F. ZimmermannJosephJ. and Oddie Karen and L. Robert and L. CooneyCharles},
  journal={Applied Biochemistry and Biotechnology},
  year={1991},
  volume={30},
  pages={137-148}
}
Heparinase was released from the periplasmic space ofFlavobacterium heparinum by three-step osmotic shock procedure. The procedure involves resuspending exponentially growing cells consecutively into (1) 40% sucrose, (2) 10 mM sodium phosphate, 2 mM magnesium chloride, pH 7, and (3) 10 mM sodium phosphate, 300 mM sodium chloride, 2 mM magnesium chloride, pH 7. Typically, 50-75% of the total heparinase activity is recovered by this procedure with an observed 7-15-fold increase in purity. The… Expand