The relative influence of phosphorylation and methylation on responsiveness of peptides to MALDI and ESI mass spectrometry.

@article{Gropengiesser2009TheRI,
  title={The relative influence of phosphorylation and methylation on responsiveness of peptides to MALDI and ESI mass spectrometry.},
  author={Jan Gropengiesser and Balamurugan T. Varadarajan and Heike Stephanowitz and Eberhard Krause},
  journal={Journal of mass spectrometry : JMS},
  year={2009},
  volume={44 5},
  pages={821-31}
}
Qualitative and quantitative analysis of post-translational protein modifications by mass spectrometry is often hampered by changes in the ionization/detection efficiencies caused by amino acid modifications. This paper reports a comprehensive study of the influence of phosphorylation and methylation on the responsiveness of peptides to matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) mass spectrometry. Using well-characterized synthetic peptide mixtures… CONTINUE READING