The relationship of KCl- and prostaglandin F2 alpha-mediated increases in tension of the porcine coronary artery with changes in intracellular Ca2+ measured with fura-2.

1. Changes in intracellular free Ca2+ concentration ([Ca2+]i) were measured simultaneously with changes in muscle tension by use of the fluorescent Ca2+ indicator, fura-2, in coronary arterial rings of the pig. 2. Changes in [Ca2+]i were measured by monitoring the ratio of fluorescence due to excitation at 340 nm (F340) to that at 380 nm (F380). 3… CONTINUE READING