## Structural Determinants of Water Permeability through the Lipid Membrane

- John C. Mathai, Stephanie Tristram-Nagle, John F. Nagle, Mark L. Zeidel
- The Journal of general physiology
- 2008

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1 Excerpt

- Published 1994 in The Journal of Membrane Biology

Permeability coefficients (P m ) across planar egg lecithin/decane bilayers and bulk hydrocarbon/water partition coefficients (K w→hc) have been measured for 24 solutes with molecular volumes, V, varying by a factor of 22 and P m values varying by a factor of 107 to explore the chemical nature of the bilayer barrier and the effects of permeant size on permeability. A proper bulk solvent which correctly mimics the microenvironment of the barrier domain was sought. Changes in P m /Kw→hc were then ascribed to size-dependent partitioning and/or size-dependent diffusivity. The diffusion coefficient-size dependency was described by D barrier = D 0 /V n . When n-decane was used as a reference solvent, the correlation between log P m /K w→hc and log V was poor (r = 0.56) with most of the lipophilic (hydrophilic) permeants lying below (above) the regression line. Correlations improved significantly (r = 0.87 and 0.90, respectively) with more polarizable solvents, 1-hexadecene and 1,9-decadiene. Values of the size selectivity parameter n were sensitive to the reference solvent (n = 0.8 ± 0.3, 1.2 ± 0.1 and 1.4 ± 0.2, respectively, for decane, hexadecene, and decadiene). Decadiene was selected as the most suitable reference solvent. The value for n in bilayer transport is higher than that for bulk diffusion in decane (n = 0.74±0.10), confirming the steep dependence of bilayer permeability on molecular size. Statistical mechanical theory recently developed by the authors suggests that a component of this steep size dependence may reside in size-dependent solute partitioning into the ordered chain region of bilayers. This theory, combined with the above diffusion model, yielded the relationship, P m /K W→hc=D 0 exp(™αV)V n . A fit of the experimental data to this model gave the best fit (r=0.93) with α = 0.0053±0.0021 and n=0.8 ± 0.3, suggesting that both diffusion and partitioning mechanisms may play a role in determining the size dependence of lipid bilayer permeabilities.

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@article{Xiang1994TheRB,
title={The relationship between permeant size and permeability in lipid bilayer membranes},
author={T. -X. Xiang and Bruce D. Anderson},
journal={The Journal of Membrane Biology},
year={1994},
volume={140},
pages={111-122}
}