The regulatory domain of protein kinase C coordinates four atoms of zinc.

@article{Quest1992TheRD,
  title={The regulatory domain of protein kinase C coordinates four atoms of zinc.},
  author={Andrew F. G. Quest and Jules Bloomenthal and Elaine S.G. Bardes and R M Bell},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 14},
  pages={10193-7}
}
Protein kinase C (PKC) was found to be a zinc metallo-enzyme. Atomic absorption measurements on the intact enzyme indicated that four zinc atoms (4.2 +/- 0.5) were bound per PKC alpha molecule. Similar stoichiometric ratios were determined for PKC beta II and PKC gamma, other PKC isoforms individually expressed in the baculovirus-insect cell expression system, as well as for purified rat brain PKC. By trypsin treatment of PKC alpha, a 32-kDa lipid binding regulatory and a 50-kDa catalytic… CONTINUE READING