The regulatory component of adenylate cyclase. Purification and properties.

@article{Sternweis1981TheRC,
  title={The regulatory component of adenylate cyclase. Purification and properties.},
  author={Paul C. Sternweis and John K. Northup and Murray Smigel and Alfred G. Gilman},
  journal={The Journal of biological chemistry},
  year={1981},
  volume={256 22},
  pages={11517-26}
}
The regulatory component (G/F) of adenylate cyclase, which has been purified previously, contains three putative subunits with molecular weights of 52,000, 45,000, and 35,000 (Northup, J. K., Sternweis, P. C., Smigel, M. D., Schleifer, L. S., Ross, E. M., and Gilman, A. G. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 6516-6520). The published procedure has been modified to reduce the time required for preparation and to increase the yield. Application of the improved procedure allows purification… CONTINUE READING

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