The regulation of total creatine content in a myoblast cell line

@article{Odoom1996TheRO,
  title={The regulation of total creatine content in a myoblast cell line},
  author={Joseph E. Odoom and Graham J. Kemp and George K. Radda},
  journal={Molecular and Cellular Biochemistry},
  year={1996},
  volume={158},
  pages={179-188}
}
Total cellular creatine content is an important bioenergetic parameter in skeletal muscle. To understand its regulation we investigated creatine transport and accumulation in the G8 cultured skeletal myoblast line. Like other cell types, these contain a creatine transporter, whose activity, measured using a radiolabelling technique, was saturable (Km = 110 ± 25 μM) and largely dependent on extracellular [Na+]. To study sustained influences on steady state creatine concentration we measured… CONTINUE READING