The regulation of haemocyanin oxygen affinity during emersion of the crayfish Austropotamobius pallipes . I. An in vitro investigation of the interactive effects of calcium and L-lactate on oxygen affinity

@inproceedings{Morris1986TheRO,
  title={The regulation of haemocyanin oxygen affinity during emersion of the crayfish Austropotamobius pallipes . I. An in vitro investigation of the interactive effects of calcium and L-lactate on oxygen affinity},
  author={S. Morris and R. Tyler-Jones and Edwin W Taylor},
  year={1986}
}
The haemolymph of the crayfish Austropotamobius pallipes exhibits a high affinity for oxygen (P50 = 2.7 Torr at pH7.9 and 15°C) and a modest Bohr effect (ϕ =0.455). The affinity of haemolymph dialysed against a crayfish Ringer was lower with a P50 value of 6.4 Torr at the same temperature and pH. The oxygen affinity of the dialysed haemolymph can be increased markedly by increased concentrations of L-lactate and to a greater extent by elevated concentrations of calcium ions. In the dialysed… CONTINUE READING

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