The reductive half-reaction of liver microsomal FAD-containing monooxygenase.

@article{Beaty1981TheRH,
  title={The reductive half-reaction of liver microsomal FAD-containing monooxygenase.},
  author={Nik Beaty and David P. Ballou},
  journal={The Journal of biological chemistry},
  year={1981},
  volume={256 9},
  pages={4611-8}
}
The addition of substrates to microsomal FAD-containing-monooxygenase (EC 1.14.13.8) is an ordered process in which FAD reduction by NADPH is necessarily the first step. This reduction by NADPH is markedly biphasic, and when analyzed for two phases, rate constants of 2.46 s-1 and 0.88 s-1 are obtained at 4 degrees C, pH 7.2. The Kd (8 microM) for the binding of NADPH to the flavoprotein prior to reduction demonstrates a tight binding. Reduction by NADH also occurs and is similar in all respects… CONTINUE READING