The reduction of water-soluble tetrazolium salt reagent on the plasma membrane of epidermal keratinocytes is oxygen dependent.

@article{Weir2011TheRO,
  title={The reduction of water-soluble tetrazolium salt reagent on the plasma membrane of epidermal keratinocytes is oxygen dependent.},
  author={Lynda Weir and Douglas Robertson and Irene M. Leigh and Andrey A Panteleyev},
  journal={Analytical biochemistry},
  year={2011},
  volume={414 1},
  pages={31-7}
}
The water-soluble tetrazolium salt (WST-1) assay is frequently used to assess cell proliferation. However, our study showed that in normal and cancerous keratinocytes, this assay is more responsive to changes in oxygenation than to rates of cell growth. Stimulation of keratinocyte proliferation by low Ca(2+) and suppression of proliferation by nocodazole resulted in modest changes in WST-1 readings, whereas gradually reducing the level of oxygen in the cellular environment from ambient (21%) to… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 49 references

Catani, Trans-plasma membrane electron transport in mammals: functional significance in health and disease, Antioxid

D. Del Principe, L. Avigliano, M.V.I. Savini
Redox Signal • 2011
View 2 Excerpts

A new source of aging?

Journal of cosmetic dermatology • 2009
View 1 Excerpt

Angiogenesis drives psoriasis pathogenesis.

International journal of experimental pathology • 2009
View 1 Excerpt

Hypoxia

B. Bedogni, M. B. Powell
melanocytes, and melanoma: survival and tumor development in the permissive microenvironment of the skin, Pigment Cell Melanoma Res. 22 • 2009
View 1 Excerpt

Low , Reactive oxygen species generation at the plasma membrane for antibody control

H. F. L. Crane
Autoimmun . Rev . • 2008

Similar Papers

Loading similar papers…