The reconstruction of a three-dimensional structure from projections and its application to electron microscopy

  title={The reconstruction of a three-dimensional structure from projections and its application to electron microscopy},
  author={R. Anthony Crowther and David J. DeRosier and Aaron Klug},
  journal={Proceedings of the Royal Society of London. A. Mathematical and Physical Sciences},
  pages={319 - 340}
A transmission electron micrograph is essentially a projection of the specimen in the direction of view. In order to reconstruct a three-dimensional image of the specimen, it is necessary to be able to combine data from a number of different views. A formal solution of this problem is given in terms of Fourier transforms. Its realization requires data reduction and interpolation. The final solution is given by a least squares approach, which also indicates how many views must be included to… Expand

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