The recent determination of the three-dimensional structure of the trrj repressor of Escherichia coli (1) and the Isolation and characterization of many classes of trp_ repressor mutants


Two small, multicopy, expression plasmids were constructed that permit convenient Insert ion of trpR. the structural gene for the t r £ repressor of Escherichja c o l l . with I t s natural rlbosome binding s i te or adjacent to the Mbosome bindTn? s i te for the t r £ leader peptide. In these plasmids trpR 1s positioned between the strong regulated ta… (More)

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