The real-time quaking-induced conversion assay for detection of human prion disease and study of other protein misfolding diseases

@article{Schmitz2016TheRQ,
  title={The real-time quaking-induced conversion assay for detection of human prion disease and study of other protein misfolding diseases},
  author={Matthias Schmitz and Maria Cramm and Franc Llorens and Dominik M{\"u}ller-Cramm and Steven John Collins and Ryuichiro Atarashi and Katsuya Satoh and Christina D Orr{\'u} and Bradley R Groveman and Saima Zafar and Walter J. Schulz-Schaeffer and Byron Caughey and Inga Zerr},
  journal={Nature Protocols},
  year={2016},
  volume={11},
  pages={2233-2242}
}
The development and adaption of in vitro misfolded protein amplification systems has been a major innovation in the detection of abnormally folded prion protein scrapie (PrPSc) in human brain and cerebrospinal fluid (CSF) samples. Herein, we describe a fast and efficient protein amplification technique, real-time quaking-induced conversion (RT-QuIC), for the detection of a PrPSc seed in human brain and CSF. In contrast to other in vitro misfolded protein amplification assays—such as protein… CONTINUE READING

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