The reaction of yeast cystathionine beta-synthase is rate-limited by the conversion of aminoacrylate to cystathionine.

@article{Jhee2001TheRO,
  title={The reaction of yeast cystathionine beta-synthase is rate-limited by the conversion of aminoacrylate to cystathionine.},
  author={Kwang Hwan Jhee and Dimitri Niks and P. Mcphie and Michael F. Dunn and Edith Wilson Miles},
  journal={Biochemistry},
  year={2001},
  volume={40 36},
  pages={
          10873-80
        }
}
Our studies of the reaction mechanism of cystathionine beta-synthase from Saccharomyces cerevisiae (yeast) are facilitated by the spectroscopic properties of the pyridoxal phosphate coenzyme that forms a series of intermediates in the reaction of L-serine and L-homocysteine to form L-cystathionine. To characterize these reaction intermediates, we have carried out rapid-scanning stopped-flow and single-wavelength stopped-flow kinetic measurements under pre-steady-state conditions, as well as… CONTINUE READING