The rational use of mass spectrometry for amino acid sequence determination in peptides and extension of the possibilities of the method

@article{Shemyakin1970TheRU,
  title={The rational use of mass spectrometry for amino acid sequence determination in peptides and extension of the possibilities of the method},
  author={M. M. Shemyakin and Y. Ovchinnikov and E. I. Vinogradova and A. Kiryushkin and M. Feigina and N. A. Aldanova and Y. Alakhov and V. Lipkin and A. I. Miroshnikov and B. V. Rosinov and S. A. Kazaryan},
  journal={FEBS Letters},
  year={1970},
  volume={7}
}
It has been shown earlier by us and by others that mass spectrometry can be used for the elucidation of the primary structure of peptides containing residues of all the ammo acids commonly found in proteins, N-acylpeptide esters being most suitable for this purpose (see [ 1,2] and references therein). Mass spectrometric determination of amino acid sequences is based on fragmentation of N-acylpeptide esters involving rupture of amide (ester) bonds and localization of the positive charge at the C… Expand
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References

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Mass spectrometry of natural and synthetic peptide derivatives
INTRODUCTION Preliminary studies on the use of mass spectrometry for the determination of amino acid sequences in peptides have been published by Biemann a al.1' 2 who reduced the peptide bonds andExpand
Primary structure determination of peptides and proteins by mass spectrometry
A pressing problem in modern organic chemistry is the determination of the structure of polypeptides and proteins, for it is here that the needs and interests of biochemistry have far surpassedExpand
Mass spectrometry of N‐permethylated peptide derivatives; artifacts produced by C‐methylation
TLDR
The Coggins-Benoiton procedure was adopted in the laboratory as the preferable method for Npermethylation, and was found to be successful for peptide sequence determination by mass spectrometry. Expand
The mass spectra of amino‐acid and peptide derivatives—III:. A comparison of the utility of various N‐protecting groups
Comparison of the acetyl-, trifluoroacetyl-, benzyloxycarbonyl-, methoxycarbonyl-, ethoxycarbonyl, methylaminocarbonyl, phenylaminocarbonyl-, phthanoyl-, and stearoyl-derivatives ofExpand
Chemical and mass spectrometric sequence studies of a peptide from the variable part of normal immunoglobulin λ‐chains
TLDR
The sequence of an octadecapeptide was independently determined on the one hand by sequential degradation (isothiocyanate method) combined with dansylation, and on the other by mass spectrometry, in fair agreement. Expand
The revised structure of the peptide antibiotic esperin, established by mass spectrometry.
TLDR
The structures of the antibiotic esperin and of esperinic acid were re-examined using the techniques of N-permethylation and mass spectrometry to find the same sequence with a lactone formed by the OH group of the fatty acid constituent and the carboxylic acid function of the aspartic acid residue. Expand
Feline gastrin. An example of peptide sequence analysis by mass spectrometry.
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