The ratio of formation of prostacyclin/thromboxane A2 in HUVEC decreased in each subsequent passage.

@article{Neubert1997TheRO,
  title={The ratio of formation of prostacyclin/thromboxane A2 in HUVEC decreased in each subsequent passage.},
  author={Katrin Neubert and Annekathrin Haberland and Ingrid Kruse and Manfred Wirth and Ingolf Schimke},
  journal={Prostaglandins},
  year={1997},
  volume={54 1},
  pages={447-62}
}
In this study, we analyzed the antioxidative potential (SOD-, GSH-Px-activity) and the basal, H2O2- and ATP-stimulated formation of PGI2 and TXA2 in human umbilical vein endothelial cells (HUVEC) of different passages. The subcultivation of cells partly represents the process of aging. Both subcultivation of the cells and the H2O2 incubation did not significantly influence the activity of SOD and GSH-Px. H2O2 (0.1 mM and 1.0 mM) stimulated the generation of PGI2 and TXA2 in the cell passages… CONTINUE READING