The ratio between CcdA and CcdB modulates the transcriptional repression of the ccd poison-antidote system.

@article{Afif2001TheRB,
  title={The ratio between CcdA and CcdB modulates the transcriptional repression of the ccd poison-antidote system.},
  author={Hassan Afif and Noureddine Allali and Martine Couturier and Laurence Van Melderen},
  journal={Molecular microbiology},
  year={2001},
  volume={41 1},
  pages={73-82}
}
The ccd operon of the F plasmid encodes CcdB, a toxin targeting the essential gyrase of Escherichia coli, and CcdA, the unstable antidote that interacts with CcdB to neutralize its toxicity. Although work from our group and others has established that CcdA and CcdB are required for transcriptional repression of the operon, the underlying mechanism remains unclear. The results presented here indicate that, although CcdA is the DNA-binding element of the CcdA-CcdB complex, the stoichiometry of… CONTINUE READING