The rate of polymerase release upon filling the gap between Okazaki fragments is inadequate to support cycling during lagging strand synthesis.

@article{Dohrmann2011TheRO,
  title={The rate of polymerase release upon filling the gap between Okazaki fragments is inadequate to support cycling during lagging strand synthesis.},
  author={Paul R Dohrmann and Carol Michelle Manhart and Christopher D Downey and Charles S McHenry},
  journal={Journal of molecular biology},
  year={2011},
  volume={414 1},
  pages={
          15-27
        }
}
Upon completion of synthesis of an Okazaki fragment, the lagging strand replicase must recycle to the next primer at the replication fork in under 0.1 s to sustain the physiological rate of DNA synthesis. We tested the collision model that posits that cycling is triggered by the polymerase encountering the 5'-end of the preceding Okazaki fragment. Probing with surface plasmon resonance, DNA polymerase III holoenzyme initiation complexes were formed on an immobilized gapped template. Initiation… CONTINUE READING

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