The rat fructose-1,6-bisphosphatase gene. Structure and regulation of expression.

  title={The rat fructose-1,6-bisphosphatase gene. Structure and regulation of expression.},
  author={M. Raafat El-Maghrabi and Alex J. Lange and Lutz K{\"u}mmel and Simon J. Pilkis},
  journal={The Journal of biological chemistry},
  volume={266 4},
The gene encoding rat fructose-1,6-bisphosphatase was isolated from a rat genomic Charon 4A library by screening with a cDNA to the rat liver enzyme. Southern blotting of rat genomic DNA showed that there is a single copy of the fructose-1,6-bisphosphatase gene. It extends over 23 kilobases and is composed of seven exons and six introns that range in size from 93 to 267 base pairs and from 1,400 to 11,300 base pairs, respectively. The intron/exon boundary sequences conform to consensus acceptor… Expand
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A cDNA encoding fructose-1,6-bisphosphatase (FBPase) was isolated from mouse liver RNA and based upon the cDNA sequence all functional parts of the mouse liver FBPase gene (including exon-intron boundaries) were PCR-amplified and sequenced. Expand
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By applying a newly developed method, cDNAs for the human muscle isoform of fructose-1,6-bisphosphatase were isolated from phage- and plasmid-derived libraries. From these cDNAs and an EST clone, aExpand
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The SV40 early promoter is active both in mammalian cells and in the fission yeast Schizosaccharomyces pombe, and is used to drive full-length cDNA in polyvalent pcD-libraries and appears to give a conclusive advantage to the rat hepatic enzyme over the human one when trans-complementing mutant yeast. Expand
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